Wilen Lab


Selected Publications


Tropism for tuft cells determines immune promotion of norovirus pathogenesis.

Science. (2018) Apr 13


Structural basis for murine norovirus engagement of bile acids and the CD300lf receptor.

Proceedings of the National Academy of Sciences. (2018) Sept 


Discovery of a proteinaceous cellular receptor for a norovirus

Science. 2016: 353(6302):933-6

All Publications

Sherman MB, Williams AN, Smith HQ, Nelson C, Wilen CB, Fremont DH, Virgin HW, SmithTJ. Bile salts alter the mouse norovirus capsid conformation; possible implications for cell attachment and immune evasion. Journal of Virology. 2019 Epub. Pubmed

Graziano VR, Wei J, Wilen CB. Norovirus Attachment and Entry. Viruses. 2019 May 30. Pubmed

Hosmillo M#, Lu J#, McAllaster MR#,  Eaglesham JB,  Wang X,  Emmott E, Domingues P,  Chaudhry Y,  Fitzmaurice TJ,  Tung MKH, Panas M, McInerney G,  Locker N, Wilen CB*, Goodfellow I*. Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation. BioRxiv. 2019. BioRxiv

Fritzlar S, Aktepe TE, Chao YW, Kenney ND, McAllaster MR, Wilen CB, White PA, Mackenzie JM. Mouse Norovirus Infection Arrests Host Cell Translation Uncoupled from the Stress Granule-PKR-eIF2α Axis. MBio. 2019 Jun 18;10(3). Pubmed

Lee S, Liu H, Wilen CB, Sychev ZE, Desai C, Hykes BL Jr, Orchard RC, McCune BT, Kim KW, Nice TJ, Handley SA, Baldridge MT, Amarasinghe GK, Virgin HW. A Secreted Viral Nonstructural Protein Determines Intestinal Norovirus Pathogenesis. Cell Host & Microbe. 2019 May 21. Pubmed

Orchard RC, Wilen CB, Virgin HW. Sphingolipid biosynthesis induces a conformational change in the murine norovirus receptor and facilitates viral infection. Nature Microbiology. 2018; 3(10):1109-1114. Pubmed

Nelson CA*, Wilen CB*, Dai YN, Orchard RC, Kim AS, Stegeman RA, Hsieh LL, Smith TJ, Virgin HW, Fremont DH. Structural basis for murine norovirus engagement of bile acids and the CD300lf receptor. Proceedings of the National Academy of Sciences of the United States of America. 2018; 115(39):E9201-E9210. Pubmed

Liu TC, Kern JT, VanDussen KL, Xiong S, Kaiko GE, Wilen CB, Rajala MW, Caruso R, Holtzman MJ, Gao F, McGovern DP, Nunez G, Head RD, Stappenbeck TS. Interactionbetween smoking and ATG16L1T300A triggers Paneth cell defects in Crohn's disease. Journal of Clinical Investigation. 2018. Pubmed

Wilen CB, Lee S, Hsieh LY, Orchard RC, Desai C, Hykes BL, McAllaster M, Balce D, Feehley T, Brestoff JR, Hickey CA, Yokoyama CC, Wang Y, MacDuff D, Kreamalmayer D, Howitt MR, Neil JA, Cadwell K, Allen PA, Handley SA, van lookeren Campagne M, Baldridge MT, Virgin HW. Tropism for tuft cells determines immune promotion of norovirus pathogenesis. Science.(2018) Apr 13. Pubmed

McMullen AR, Wilen CB, Burnham CAD. Epidemiology of Blood Stream Infections in the Dark Art of Blood Culture.  Dunne M and Burnham CAD, Editors. ASM press. (2018). Link

Lee S, Wilen CB, Orvedahl A, McCune BT, Kim KW, Orchard RC, Peterson ST, Nice TJ, Baldridge MT, Virgin HW. Norovirus cell tropism is determined by combinatorial action of a viral non-structural protein and host cytokine. Cell Host & Microbe. (2017) Oct 11. Pubmed

Biering SB,Choi J, Halstrom RA, Brown HM, Beatty WL, Lee S, McCune BT, Dominici E, Williams LE, Orchard RC, Wilen CB, Yamamoto M, Coers J, Taylor GA, and Hwang S. Viral replication complexes are targeted by LC3-guided interferon-inducible GTPases. Cell Host & Microbe. (2017) Jul 12;22(1):74-85. Pubmed

Wilen CB, Booth GS, Grossman BJ, Lane WJ, Szlarski PC, Jackups R. Using direct antiglobulin test results to reduce unnecessary cold agglutinin testing. Transfusion.(2017) Mar 7; 57(6): 1480-4. Pubmed

Orchard RC*, Wilen CB*, Doench JG, Baldridge MT, McCune BT, Lee YJ, Lee S, Pruett-Miller SM, Nelson CA, Fremont DH, and Virgin HW. Discovery of a proteinaceous cellular receptor for a norovirus. Science.2016: 353(6302):933-6. Pubmed

O’Brien M, Manches O, Wilen CB, Wu V, Sunseri N, Bhardwaj N. CD4 receptor is key determinant of divergent HIV-1 sensing by plasmacytoid dendritic cells (2016) PLoSPathogens. (2016) Apr 15;12(4)e1005553. Pubmed

Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova, Wilen CB, Orchard RC, Virgin HW, Listgarten J, and Root DE.  Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nature Biotechnology. (2016) 34(2):184-91. Pubmed

Monaco CL, Gootenberg DB, Zhao G, Handley SA, Ghebremichael MS, Lim ES, Lankowski A, Baldridge MT, Wilen CB, Flagg M, Norman JM, Keller BC, Levano JM, Wang D, Boum Y, Martin JN, Hunt PW, Bangsberg DR, Siedner MJ, Kown DS, and Virgin HW. Altered Virome and Bacterial Microbiome in Human Immunodeficiency Virus-associated Acquired Immunodeficiency Syndrome. Cell Host & Microbe(2016) 19(3): 311-22. Pubmed

Lu Q, Yokoyama CC, Williams JW, Baldridge MT, Jin X, DesRochers B, Bricker T, Wilen CB, Bagaitkar J, Loginicheva E, Sergushichev A, Kreamalmeyer D, Keller BC, Zhao Y, Kambal A, Green DG, Martinez J, Dinauer MC, Holtzman MJ, Crouch EC, Beatty W, Boon ACM, Zhang H, Randolph GJ, Artyomov MN, and Virgin HW. Homeostatic control of innate immune lung inflammation by Vici syndrome gene Epg5and additional autophagy genes promotes influenza virus pathogenesis.  Cell Host & Microbe(2016) 19(1):102-13. Pubmed

Gonzalez MD*, Wilen CB*, and Burnham CAD. Markers of intestinal inflammation for the diagnosis of infectious gastroenteritis. (2015) Clin Lab Med. 34(2): 333-44. Pubmed

Wilen CB, McMullen AR, and Burnham CAD. Comparison of sample preparation methods, instrumentation platforms, and contemporary commercial databases for identification of clinically relevant mycobacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry. (2015) J Clin Micro. 53(7): 2308-15. Pubmed

Wilen CB, Szymanski JJ, Hung S, Rajan A, Lavigne PM, Char DM, Geltman EM, and Scott MG. The impact on patient management and outcome of switching between two contemporary sensitive cardiac troponin assays. (2015) Clin Chem. 61(6):870-6. Pubmed

Wilen CB, Monaco CM, Hoppe-Bauer J, Jackups R, Bucelli RC, Burnham CAD.  Criteria to reduce unnecessary testing for herpes simplex virus, varicella-zoster virus, cytomegalovirus, and enterovirus in cerebrospinal fluid samples from adults. (2015) J Clin Micro.52(3): 887-95.  Pubmed

Wilen CB, Orner C, Brown SM. Sustaining Point of Care Testing in a Resource-Limited Environment: Experiences in Hispaniola, in Global Point-of-Care Strategies for Disasters, Complex Emergencies, and Public Health Crises: Enhancing Standards of Care at the Site of Need.Kost GJ and Curtis CM, editors. 2014, AACC Press: Washington, DC.

Petersen J*, Drake MJ*, Bruce EA*, Riblett AM, Didigu CA, Wilen CB, Malani N, Male F, Lee FH, Bushman FD, Cherry S, Doms RW, Bates P, Briley K Jr. The major cellular sterol regulatory pathway is required for andes virus infection. (2014) PLoS Pathogens 10(2):e1003911. Pubmed

Didigu CA, Wilen CB, Wang J, Duong J, Secreto AJ, Danet-Desnoyers GA, Riley JL, Gregory PD, June CH, Holmes MC, Doms RW. Simultaneous zinc-finger nuclease editing of the HIV coreceptors ccr5 and cxcr4 protects CD4+ T cells from HIV-1 infection. (2014) Blood123(1): 61-9. Pubmed

Parrish NF, Gao F, Li H, Giorgi EE, Barbian HJ, Parrish EH, Zajic L, Iyer SS, Decker JM, Kumar A, Hora B, Berg A, Cai F, Hopper J, Denny TN, Ding H, Ochsenbauer C, Kappes JC, Galimidi RP, West AP Jr, Bjorkman PJ, Wilen CB, Doms RW, O'Brien M, Bhardwaj N, Borrow P, Haynes BF, Muldoon M, Theiler JP, Korber B, Shaw GM, Hahn BH. Phenotypic properties of transmitted founder HIV-1.  (2013) Proc Natl Aca Sci110(17):6626-33.  Pubmed

Parker ZF*, Iyer SS*, Wilen CB, Parrish NF, Chikere KC, Lee F, Didigu CA, Berro R, Klasse PJ, Lee B, Moore JP, Shaw GM, Hahn BH, and Doms RW. Transmitted/Founder and Chronic HIV-1 Envelope Proteins are Distinguished by Differential Utilization of CCR5. (2013) J Virol 87(5):2401-11. Pubmed

Parrish NF*, Wilen CB*, Banks L, Iyer S, Pfaff JM, Salazar-Gonzalez JF, Salazar MG, Decker JM, Parrish EH, Berg A, Hopper J, Kumar A, Mahlokozera T, Yuan S, Coleman C, Vermeulen M, Ochsenbauer C, Tilton JC, Permar SR, Kappes JC, Betts MR, Busch MP, Gao F, Montefiori D, Haynes BF, Shaw GM, Hahn BH, Doms RW.  Transmitted/founder and chronic subtype C HIV-1 use CD4 and CCR5 receptors with equal efficiency and are not inhibited by blocking the integrin α4β7.  (2012) PLoS Pathogens. 8(5):e1002686. Pubmed

Wilen CB, Tilton JC, Doms RW.  HIV: cell binding and entry, in Human Immunodeficiency Virus. FD Bushman, G Nabel, and R Swanstrom, Editors. 2012, Cold Spring Harbor: Cold Spring Laboratory Press:  Cold Spring Harbor, NY. Pubmed

Wilen CB, Tilton JC, and Doms RW.  Molecular mechanisms of HIV entry, in Viral Molecular Machines, M. Rossman and V. Rao, Editors. 2012, Springer-Verlag: New York, NY. Pubmed

Jiang C*, Parrish N*, Wilen CB*, Li H*, Chen Y, Pavlicek JW, Berg A, Tomaras G, Tilton JC, Decker JM, Haynes BF, Shaw GM, Doms RW, Hahn BH, Gao F. Primary infection by a human immunodeficiency virus with atypical coreceptor tropism. (2011) J Virol 85(20):10669-81Pubmed

Wilen CB*, Parrish N*, Pfaff JM, Decker JM, Henning EA, Haynes BF, Tilton JC, Shaw GM, Hahn BH, Doms RW. Phenotypic comparison of clade B transmitted/founder and chronic HIV-1 Envs. (2011) J Virol 85(17):8514-27.Pubmed

Wilen CB, Wang J, Tilton JC, Miller JC, Kim KA, Rebar EJ, Sherrill-Mix SA, Patro SC, Secreto AJ, Jordan APO, Lee G, Kahn J, Aye PP, Bunnell BA, Lackner AA, Hoxie JA, Danet-Desnoyers GA, Bushman FD, Riley JL, Gregory PD, June CH, Holmes MC, Doms RW. Engineering HIV-resistant human CD4+ T cells with CXCR4-specific zinc finger nucleases. (2011) Plos Pathogens7(4):e1002020. Pubmed

Tilton, JC, Wilen CB, Didigu C, Sinha R, Harrison J, Agrawal-Gamse C, Henning E, Bushman FD, Deeks SD, Doms RW.  A maraviroc-resistant HIV-1 with narrow cross-resistance to other CCR5 antagonists depends on both N-terminal and extracellular loop domains of drug-bound CCR5. (2010) J Virol 84(20):10863-76.Pubmed

Leung W, Shaffer CD, Cordonnier T, Wong J, Itano MS, Slawson Tempel EE, Kellmann E, Desruisseau DM, Cain C, Carrasquillo R, Chusak TM, Falkowska K, Grim KD, Guan R, Honeybourne J, Khan S, Lo L, McGaha R, Plunkett J, Richner JM, Richt R, Sabin L, Shah A, Sharma A, Singhal S, Song F, Swope C, Wilen CB, Buhler J, Mardis ER, Elgin SC. Evolution of a distinct genomic domain in Drosophila: comparative analysis of the dot chromosome in Drosophila melanogaster and Drosophila virilis. (2010) Genetics 185(4):1519-34. Pubmed

Pfaff JM, Wilen CB, Harrison JE, Demarest JF, Lee B, Doms RW, Tilton JC.  HIV-1 resistance to CCR5 antagonists associated with highly efficient use of CCR5 and altered tropism on primary CD4+ T cells. (2010) J Virol. 84(13):6505-14. Pubmed

*These authors contributed equally